Ascorbate transport and recycling by SH-SY5Y neuroblastoma cells: Response to glutamate toxicity

被引:49
作者
May, James M. [1 ]
Li, Liying [1 ]
Hayslett, Kendra [1 ]
Qu, Zhi-chao [1 ]
机构
[1] Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA
关键词
ascorbate transport; dehydroascorbate reduction; glutamate uptake; excitotoxicity; GSH; oxidant stress; SH-SY5Y; neuroblastoma cells;
D O I
10.1007/s11064-006-9077-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neurons maintain relatively high intracellular concentrations of vitamin C, or ascorbic acid. In this work we studied the mechanisms by which neuronal cells in culture transport and maintain ascorbate, as well as how this system responds to oxidant stress induced by glutamate. Cultured SH-SY5Y neuroblastoma cells took up ascorbate, achieving steady-state intracellular concentrations of 6 mM and higher at extracellular concentrations of 200 mu M and greater. This gradient was generated by relatively high affinity sodium-dependent ascorbate transport (K-m of 113 mu M). Ascorbate was also recycled from dehydroascorbate, the reduction of which was dependent on GSH, but not on D-glucose. Glutamate in concentrations up to 2 mM caused an acute concentration-dependent efflux of ascorbate from the cells, which was prevented by the anion channel blocker 4,4 '-diisothiocyanatostilbene-2,2 '-disulfonic acid. Intracellular ascorbate did not affect radiolabeled glutamate uptake, showing absence of heteroexchange.
引用
收藏
页码:785 / 794
页数:10
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