Multipotent adult progenitor cell isolation and culture procedures

被引:58
作者
Breyer, April [1 ]
Estharabadi, Nima [1 ]
Oki, Masayuki [1 ]
Ulloa, Fernando [1 ]
Nelson-Holte, Molly [1 ]
Lien, Laura [1 ]
Jiang, Yuehua [1 ]
机构
[1] Univ Minnesota, Stem Cell Inst, Minneapolis, MN 55455 USA
关键词
TRANSCRIPTION FACTOR; POU-DOMAIN; STEM-CELLS; PLURIPOTENCY; NANOG;
D O I
10.1016/j.exphem.2006.07.013
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed. (c) 2006 International Society for Experimental Hematology. Published by Elsevier Inc.
引用
收藏
页码:1596 / 1601
页数:6
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