Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius

被引：93

作者：

Maruta, K

Mitsuzumi, H

Nakada, T

Kubota, M

Chaen, H

Fukuda, S

Sugimoto, T

Kurimoto, M

机构：

[1] Hayashibara Biochem. L., Okayama 700

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1996年 / 1291卷 / 03期

关键词：

trehalose; maltooligosyltrehalose; gene cloning; nucleotide sequence; (Sulfolobus);

D O I：

10.1016/S0304-4165(96)00082-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trehalose biosynthesis genes, treZ, treX and treY, encoding maltooligosyltrehalose trehalohydrolase (TreZ), glycogen debranching enzyme (TreX), and maltooligosyltrehalose synthase (TreY) have been cloned from the thermophilic archaebacterium Sulfolobus acidocaldarius ATCC33909. The amino-acid sequences deduced from treZ, treX and treY are composed of 556, 713 and 720 amino-acid residues, respectively. TreZ and TreY are 33-40% homologous to the corresponding enzymes from Arthrobacter sp. Q36. We have proposed that the biosynthesis of trehalose in Sulfolobus occurs via the actions of the three enzymes encoded by treZXY.

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页码：177 / 181

页数：5

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