Epidermal localization and protective effects of topically applied superoxide dismutase

被引：13

作者：

Filipe, P

Emerit, I

Vassy, J

Rigaut, JP

Martin, E

Freitas, J

Fernandes, A

机构：

[1] UNIV PARIS 06,INST BIOMED CORDELIERS,OXYGEN FREE RAD RES GRP,PARIS,FRANCE

[2] CNRS,PARIS,FRANCE

[3] UNIV PARIS 07,HOP ST LOUIS,INST HEMATOL,LAB ANAL IMAGES PATHOL CELLULAIRE,F-75221 PARIS 05,FRANCE

[4] HOP KREMLIN BICETRE,SERV ANATOMOPATHOL,LE KREMLIN BICETR,FRANCE

[5] HOSP SANTA MARIA,DEPT DERMATOL,LISBON,PORTUGAL

[6] FAC MED,CTR METAB & ENDOCRINOL,LISBON,PORTUGAL

来源：

EXPERIMENTAL DERMATOLOGY | 1997年 / 6卷 / 03期

关键词：

SOD-FITC; epidermis; confocal epifluorescence microscopy; PUVA; human skin;

D O I：

10.1111/j.1600-0625.1997.tb00157.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Data from the literature, as well as our previous work, indicate a protective effect of superoxide dismutase (SOD) in topical application against UV-induced cutaneous damage. In the present article we show that pre-treatment of the skin with SOD protects against PUVA-induced inflammatory reactions not only in murine, but also in human skin. Using fluorescently labelled Cu,Zn SOD, epifluorescence microscopy and digital Image processing, we demonstrate that the FITC fluorescence localizes in the stratum corneum and upper granulosa, as well as in the epidermal cell layer surrounding the lumina of the hair follicles. These findings were similar for murine and human skin. Since autofluorescence was eliminated by a special filter, it can be ascertained that the fluorescence observed in the tissues was due to FITC-labelled SOD.

引用

页码：116 / 121

页数：6