Cloning and characterization of a regulatory gene of the SARP family and its flanking region from Streptomyces ambofaciens

被引：5

作者：

Culebras, E ^{[1
]}

Martínez, E ^{[1
]}

Carnero, A ^{[1
]}

Malpartida, F ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Ctr Nacl Biotecnol, CSIC, E-28049 Madrid, Spain

来源：

MOLECULAR AND GENERAL GENETICS | 1999年 / 262卷 / 4-5期

关键词：

antibiotic; bldA; thioesterase; transcriptional regulator; polyketide biosynthesis;

D O I：

10.1007/s004380051135

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a search for activators of secondary metabolism we isolated a 12.6-kb DNA fragment from a genomic library of Streptomyces ambofaciens NRRL 2240 (the spiramycin producer). Sequencing of 6 kb of the cloned fragment revealed a cluster of four ORFs (ORF1-4) whose deduced products showed similarities to those of other genes involved in polyketide biosynthesis, including a pathway-specific regulatory gene of the SARP family. The results of insertional inactivation of some of the cloned genes clearly indicate that the isolated cluster does not code for spiramycin production, suggesting that some other polyketide compound might well be produced by this strain.

引用

页码：730 / 737

页数：8

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