Gating and modulation of presumptive NaV1.9 channels in enteric and spinal sensory neurons

被引：102

作者：

Coste, B ^{[1
]}

Osorio, N ^{[1
]}

Padilla, F ^{[1
]}

Crest, M ^{[1
]}

Delmas, P ^{[1
]}

机构：

[1] CNRS, Integrat Informat Sensorielles, UMR 6150, Fac Med,IFR Jean Roche, F-13916 Marseille 20, France

来源：

MOLECULAR AND CELLULAR NEUROSCIENCE | 2004年 / 26卷 / 01期

关键词：

D O I：

10.1016/j.mcn.2004.01.015

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The Na(V)1.9 subunit is expressed in nociceptive dorsal root ganglion (DRG) neurons and sensory myenteric neurons in which it generates 'persistent' tetrodotoxin-resistant (TTX-R) Na+ currents of yet unknown physiological functions. Here, we have analyzed these currents in details by combining single-channel and whole-cell recordings from cultured rat DRG and myenteric neurons. Comparison of single-channel with whole-cell data indicates that recording using internal CsCl best reflects the basic electrical features of Na(V)1.9 currents. Inclusion of fluoride in the pipette solution caused a negative shift in the activation and inactivation gates of Na(V)1.9 but not Na(V)1.8. Fluoride acts by promoting entry of Na(V)1.9 channels into a preopen closed state, which causes a strong bias towards opening and enhances the ability of sensory neurons to sustain spiking. Thus, the modulation of the resting-closed states of Na(V)1.9 channels strongly influences nociceptor excitability and may provide a mechanism by which inflammatory mediators alter pain threshold. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：123 / 134

页数：12

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