hnRNP-U is a specific DNA-dependent protein kinase substrate phosphorylated in response to DNA double-strand breaks

被引：30

作者：

Berglund, Fredfk M. ^{[1
]}

Clarke, Paul R. ^{[1
]}

机构：

[1] Univ Dundee, Biomed Res Inst, Coll Med Dent & Nursing, Ninewells Hosp & Med Sch, Dundee DD1 9SY, Scotland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 01期

关键词：

DNA-PK; hnRNP-U; SAF-A; DNA damage; Etoposide; MESSENGER-RNA; ATM; PK; CHK1; IDENTIFICATION; CHECKPOINT; ACTIVATION; INHIBITOR; RADIATION; MUTATION;

D O I：

10.1016/j.bbrc.2009.02.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellular responses to DNA damage are orchestrated by the large phosphoinositol-3-kinase related kinases ATM, ATR and DNA-PK. We have developed a cell-free system to dissect the biochemical mechanisms of these kinases. Using this system, we identify heterogeneous nuclear ribonucleoprotein U (hnRNP-U), also termed scaffold attachment factor A (SAF-A), as a specific substrate for DNA-PK. We show that hnRNP-U is phosphorylated at Ser59 by DNA-PK in vitro and in cells in response to DNA double-strand breaks. Phosphorylation of hnRNP-U suggests novel functions for DNA-PK in the response to DNA damage. (C) 2009 Elsevier Inc. All rights reserved.

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收藏

页码：59 / 64

页数：6

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