Quantitative fluorescence microscopy: From art to science

被引：67

作者：

Fricker, Mark ^{[1
]}

Runions, John

Moore, Ian

机构：

[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England

[2] Oxford Brookes Univ, Sch Biol & Mol Sci, Oxford OX3 0BP, England

来源：

ANNUAL REVIEW OF PLANT BIOLOGY | 2006年 / 57卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

confocal scanning laser microscopy; green fluorescent protein; biosensors; cameleon; live-cell imaging;

D O I：

10.1146/annurev.arplant.57.032905.105239

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A substantial number of elegant experimental approaches have been developed to image the distribution and dynamics of DNA, mRNA, proteins, organelles, metabolites, and ions in living plant cells. Although the human brain can rapidly assimilate visual information, particularly when presented as animations and movies, it is much more challenging to condense the phenomenal amount of data present in three-, four-, or even five-dimensional images into statistically useful measurements. This review explores a range of in vivo fluorescence imaging applications in plants, with particular emphasis on where quantitative techniques are beginning to emerge.

引用

页码：79 / 107

页数：29

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