Production of dextransucrase, dextran and fructose from sucrose using Leuconostoc mesenteroides NRRL B512(f)

被引:78
作者
Santos, M [1 ]
Teixeira, J [1 ]
Rodrigues, A [1 ]
机构
[1] Univ Porto, Fac Engn, Lab Separat & React Engn, P-4099 Porto, Portugal
关键词
Leuconostoc mesenteroides; dextransucrase; dextran; fructose; batch fermentation; kinetic; modelling;
D O I
10.1016/S1369-703X(99)00047-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of dextransucrase, dextran and fructose by sucrose fermentation using Leuconostoc mesenteroides NRRL-B512(F) was studied in batch operation in a bioreactor with total working volume of 1.5 dm(3). The effect of temperature (20 to 40 degrees C), pH (5.5 and 6.7) and sucrose concentration (10 to 120 g/l) on process performance was studied. The optimum conditions for dextran and fructose production were T = 35 degrees C and pH = 5.5. Cell growth is not inhibited by high sucrose concentrations; however, for sucrose concentration higher than 40 g/dm(3) separation of products from cells is difficult. Biomass (X), enzyme (E), dextran (D), fructose (F) and sucrose (S) rate equations were considered in order to derive a simple fermentation kinetic model from batch experimental data. The logistic equation provided a reasonable description for cell concentration, X. The Luedeking and Piret equation was used to describe the enzyme production rate, by considering only the growth associated term. The concentrations of products (dextran and fructose) were reasonably described by a first order kinetic law with respect to both substrate and enzyme concentrations; the substrate, S was consumed for cell growth and for dextran and fructose production. Model parameters mu(m) and X-0 were calculated from cell growth as a function of time. The yield Y-E/X were calculated from X-max and E-max and Y-x/s was estimated from X-max and the sucrose consumed by the bacteria. The remaining parameter k' was obtained by fitting the experimental data of substrate, dextran and fructose concentrations versus time. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:177 / 188
页数:12
相关论文
共 20 条
[1]  
ALSOP RM, 1983, PROGR IND MICROBIOL, V18, P1
[2]  
BARKER PE, 1993, J CHEM TECHNOL BIOT, V57, P21
[3]   THE PRODUCTION OF THE ENZYME DEXTRANSUCRASE USING NONAERATED FERMENTATION TECHNIQUES [J].
BARKER, PE ;
AJONGWEN, NJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (08) :703-707
[4]  
BROWN DE, 1989, J CHEM TECHNOL BIOT, V48, P405
[5]  
DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x
[6]   STUDIES ON THE AEROBIC CARBOHYDRATE METABOLISM OF LEUCONOSTOC-MESENTEROIDES [J].
JOHNSON, MK ;
MCCLESKEY, CS .
JOURNAL OF BACTERIOLOGY, 1957, 74 (01) :22-25
[7]   Large-scale preparation of highly purified dextransucrase from a high-producing constitutive mutant of Leuconostoc mesenteroides B-512FMC [J].
Kitaoka, M ;
Robyt, JF .
ENZYME AND MICROBIAL TECHNOLOGY, 1998, 23 (06) :386-391
[8]   Use of a microtiter plate screening method for obtaining Leuconostoc mesenteroides mutants constitutive for glucansucrase [J].
Kitaoka, M ;
Robyt, JF .
ENZYME AND MICROBIAL TECHNOLOGY, 1998, 22 (06) :527-531
[9]   EXTRACELLULAR MICROBIAL POLYSACCHARIDES - KINETICS OF PSEUDOMONAS SP, AZOTOBACTER-VINELANDII, AND AUREOBASIDIUM-PULLULANS BATCH FERMENTATIONS [J].
KLIMEK, J ;
OLLIS, DF .
BIOTECHNOLOGY AND BIOENGINEERING, 1980, 22 (11) :2321-2342
[10]   ENZYMATIC SYNTHESIS OF DEXTRAN [J].
KOEPSELL, HJ ;
TSUCHIYA, HM .
JOURNAL OF BACTERIOLOGY, 1952, 63 (02) :293-295