Use of a microtiter plate screening method for obtaining Leuconostoc mesenteroides mutants constitutive for glucansucrase

被引：23

作者：

Kitaoka, M ^{[1
]}

Robyt, JF ^{[1
]}

机构：

[1] Iowa State Univ Sci & Technol, Dept Biochem & Biophys, Lab Carbohydrate Chem & Enzymol, Ames, IA 50011 USA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1998年 / 22卷 / 06期

关键词：

Enzyme; screening; microtiter plate; constitutive mutant; Leuconostoc mesenteroides; glucansucrase; dextransucrase; alternansucrase; dextran; alternan;

D O I：

10.1016/S0141-0229(97)00252-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new method using microtiter plates for screening mutants for the production of glucansucrases of Leuconostoc mesenteroides was developped. The method significantly increased the efficiency of the screening process for enzyme production of mutants. Several high-producing constitutive mutants for glucansucrase were selected from L. mesenteroides B-512FMC, B-742CB, B-1299CB, and B-1355C. Enzyme production of the mutants were stable for at least 6 months with culture transfer energy two weeks and as lyophilized cultures. The method has wide-ranging capabilities beyond the screening for glucansucrase-producing mutants and it could be used for the screening of many kinds of enzymes or compounds produced by microbial mutants. (C) 1998 Elsevier Science Inc.

引用

页码：527 / 531

页数：5

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