PRODUCTION AND SELECTION OF MUTANTS OF LEUCONOSTOC-MESENTEROIDES CONSTITUTIVE FOR GLUCANSUCRASES

被引：67

作者：

KIM, D ^{[1
]}

ROBYT, JF ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT BIOCHEM & BIOPHYS,CARBOHYDRATE CHEM & ENZYMOL LAB,AMES,IA 50011

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 08期

关键词：

CONSTITUTIVE MUTANT; LEUCONOSTOC MESENTEROIDES B-512FMC; GLUCANSUCRASE; DEXTRANSUCRASE CONSTITUTIVE MUTANT; DEXTRANSUCRASE SUBUNITS; DEXTRAN STIMULATION; DEXTRANSUCRASE DEXTRAN BINDING;

D O I：

10.1016/0141-0229(94)90086-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

After chemical mutagenesis using ethyl methane sulfonate, we isolated mutants constitutive for glucansucrases from Leuconostoc mesenteroides NRRL B-512FM, B-1142, and B-1355. Those mutants produced glucansucrases when grown on D-glucose as well as on sucrose. They produced higher glucansucrase activities (3 to 22 times) when grown on D-glucose than the parent strains grown on sucrose. Glucansucrases from mutants B-1355C and B-1142C grown on glucose formed glucans that were highly resistant to Penicillium dextranase hydrolysis. Mutant B-512FMC dextransucrase formed the same kind of dextran as the parent strain; however, it showed higher thermal stability even when dextran was absent.

引用

页码：659 / 664

页数：6

共 26 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF AN EXTRACELLULAR GLUCANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES NRRL B-1355 THAT SYNTHESIZES AN ALTERNATING (1-]6), (1-]3)-ALPHA-D-GLUCAN [J].

COTE, GL ;

ROBYT, JF .

CARBOHYDRATE RESEARCH, 1982, 101 (01) :57-74

[2] THE USE OF IMMOBILIZED CONCANAVALIN A FOR THE SEPARATION OF ALTERNANSUCRASE FROM DEXTRANSUCRASE IN CULTURE BROTH OF LEUCONOSTOC MESENTEROIDES NRRL B-1355 [J].

Cote, Gregory L. .

BIOTECHNOLOGY TECHNIQUES, 1992, 6 (01) :45-48

[3]

DAY DF, 1992, ANN NY ACAD SCI, V672, P573

[4]

EBERT KH, 1962, Z NATURFORSCH PT B, VB 17, P732

[5]

GROENWALL AJT, 1945, ACTA PHYSL SCAND, V9, P1

[6]

GROENWALL AJT, 1944, ACTA PHYSL SCAND, V7, P97

[7]

HARDIN B, 1991, AGR RES, V39, P27

[8] CHARACTERIZATION AND CLASSIFICATION OF DEXTRANS FROM 96 STRAINS OF BACTERIA [J].

JEANES, A ;

HAYNES, WC ;

WILHAM, CA ;

RANKIN, JC ;

MELVIN, EH ;

AUSTIN, MJ ;

CLUSKEY, JE ;

FISHER, BE ;

TSUCHIYA, HM ;

RIST, CE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1954, 76 (20) :5041-5052

[9] UNUSUAL DEXTRANS .7. ALPHA-D-GLUCOPYRANOSIDIC LINKAGES OF DEXTRANS - COMPARISON OF PERCENTAGES FROM STRUCTURAL-ANALYSIS BY PERIODATE-OXIDATION AND BY METHYLATION [J].

JEANES, A ;

SEYMOUR, FR .

CARBOHYDRATE RESEARCH, 1979, 74 (SEP) :31-40

[10] CHARACTERIZATION OF THE MULTIPLE FORMS AND MAIN COMPONENT OF DEXTRANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES NRRL B-512F [J].

KOBAYASHI, M ;

MATSUDA, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 614 (01) :46-62

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