CONSTITUTIVE MUTANTS FOR DEXTRANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES NRRL B-512F

被引：13

作者：

MIZUTANI, N ^{[1
]}

YAMADA, M ^{[1
]}

TAKAYAMA, K ^{[1
]}

SHODA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 77卷 / 03期

关键词：

D O I：

10.1016/0922-338X(94)90228-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Constitutive mutants for dextransucrase were isolated from cells of Leuconostoc mesenteroides NRRL B-512F by treatment with N-methyl-N'-nitro-N-nitrosoguanidine, growing on an agar plate containing glucose as a carbon source and overlaying a soft agar with sucrose and tetracycline. These mutants were able to produce the enzyme in a liquid media containing sugars other than sucrose, such as glucose, fructose and maltose, without simultaneous synthesis of dextran. The enzyme activity of one mutant strain, SH 3002, was 2- to 3-fold higher than that of the wild strain grown on sucrose. When the concentration of glucose in the medium was increased from 2 to 4%, a 1.7-fold increase of enzyme activity was obtained for the mutant, whereas only a slight increase of the activity was observed on sucrose for both the wild strain and the mutant.

引用

页码：248 / 251

页数：4

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