Transient increase of a protein kinase activity identified to CK2 during sea urchin development

被引：19

作者：

Delalande, C ^{[1
]}

Bellé, R ^{[1
]}

Cormier, P ^{[1
]}

Mulner-Lorillon, O ^{[1
]}

机构：

[1] Univ Paris 06, Stn Biol Roscoff, UFR 937, CNRS,UPR 9042,Inst Natl Sci Univers, F-29682 Roscoff, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 266卷 / 02期

关键词：

casein kinase 2; development; sea urchin; hatching; blastula;

D O I：

10.1006/bbrc.1999.1842

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using GST-EF-1 delta as an exogenous substrate, and EF-1 delta kinase activity was shown to increase transiently during early development of sea urchin embryos. The basal activity of EF-1 delta kinase in unfertilized eggs was 150 fmoles/min/mg protein. The activity began to increase 10 h after fertilization and reached its maximum level (8.4 x basal) at 24 h, The activity then declined to twice the basal value at 72 h postfertilization. The EF-1 delta kinase activity was identified to a CK2-type enzyme on the basis of its substrate specificity for EF-1 delta, crude casein and beta casein, its inhibition by heparin, DRB, 2,3-bisphosphoglycerate, and its stimulation by spermine, spermidine, and polylysin, Furthermore, the activity was inhibited by the synthetic peptide RRREEETEEE specific for CK2, DRB (200 mu M) and 2,3-bisphosphoglycerate (2.5 mM) blocked or delayed the transition from blastula to gastrula of the embryos, suggesting a role for the kinase in early development. (C) 1999 Academic Press.

引用

页码：425 / 431

页数：7