Method for functional study of mitochondria in rat hypothalamus

被引：12

作者：

Benani, Alexandre ^{[1
]}

Barquissau, Valentin ^{[1
]}

Carneiro, Lionel ^{[1
]}

Salin, Benedicte ^{[2
]}

Colombani, Anne-Laure ^{[1
]}

Leloup, Corinne ^{[1
]}

Casteilla, Louis ^{[1
]}

Rigoulet, Michel ^{[2
]}

Penicaud, Luc ^{[1
]}

机构：

[1] Univ Toulouse 3, CNRS, UMR Metab Plasticite Mitochondrie 5241, F-31432 Toulouse, France

[2] Univ Bordeaux 2, Inst Biochim & Genet Cellulaires, CNRS, UMR 5095, F-33076 Bordeaux, France

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2009年 / 178卷 / 02期

关键词：

Brain; Hypothalamus; Mitochondria; Respiration; Metabolism; Oxidative phosphorylation; HIGH RESPIRATORY CONTROL; OXIDATIVE-PHOSPHORYLATION; ENERGY-METABOLISM; CULTURED NEURONS; MUSCLE-FIBERS; IN-SITU; ASTROCYTES; CHAIN; MECHANISM; SUPPORT;

D O I：

10.1016/j.jneumeth.2008.12.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Different roles of mitochondria in brain function according to brain area are now clearly emerging. Unfortunately, no technique is yet described to investigate mitochondria function in specific brain area. In this article, we provide a complete description of a procedure to analyze the mitochondrial function in rat brain biopsies. Our two-step method consists in a saponin permeabilization of fresh brain tissues in combination with high-resolution respirometry to acquire the integrated respiratory rate of the biopsy. In the first part, we carefully checked the mitochondria integrity after permeabilization, defined experimental conditions to determine the respiratory control ratio (RCR), and tested the reproducibility of this technique. In the second part, we applied our method to test its sensitivity. As a result, this method was sensitive enough to reveal region specificity of mitochondrial respiration within the brain. Moreover, we detected physiopathological modulation of the mitochondrial function in the hypothalamus. Thus this new technique that takes all cell types into account, and does not discard or select any mitochondria sub-population is very suitable to analyze the integrated mitochondrial respiration of brain biopsies. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：301 / 307

页数：7

共 42 条

[11] A central thermogenic-like mechanism in feeding regulation: An interplay between arcuate nucleus T3 and UCP2 [J].