The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells

被引：521

作者：

Banerjee, Akhilesh ^{[1
]}

Arha, Manish ^{[1
]}

Choudhary, Soumitra ^{[2
]}

Ashton, Randolph S. ^{[3
,4
]}

Bhatia, Surita R. ^{[2
]}

Schaffer, David V. ^{[3
,4
]}

Kane, Ravi S. ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Howard P Isermann Dept Chem & Biol Engn, Troy, NY 12180 USA

[2] Univ Massachusetts, Dept Chem Engn, Amherst, MA 01003 USA

[3] Univ Calif Berkeley, Dept Chem Engn, Dept Bioengn, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

来源：

BIOMATERIALS | 2009年 / 30卷 / 27期

关键词：

Alginate; Hydrogel; Modulus; Stem cells; Proliferation; Differentiation; SPINAL-CORD-INJURY; ALGINATE HYDROGELS; PHYSICAL-PROPERTIES; IN-VITRO; MATRIX; SUBSTRATE; MICROENVIRONMENTS; RESPONSES; CULTURE; BEADS;

D O I：

10.1016/j.biomaterials.2009.05.050

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

There has been an increasing interest in understanding how the mechanical properties of the microenvironment influence stem cell fate. We describe studies of the proliferation and differentiation neural stem cells (NSCs) encapsulated within three-dimensional scaffolds - alginate hydrogels - whose elastic moduli were varied over two orders of magnitude. The rate of proliferation of neural stem cells decreased with increase in the modulus of the hydrogeis. Moreover, we observed the enhancement in expression of the neuronal marker beta-tubulin III within the softest hydrogels, which had an elastic modulus comparable to that of brain tissues. To our knowledge, this work represents the first demonstration of the influence of modulus on NSC differentiation in three-dimensional scaffolds. Three-dimensional scaffolds that control stem cell fate would be broadly useful for applications in regenerative medicine and tissue engineering. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4695 / 4699

页数：5

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