Stomatal conductance does not correlate with photosynthetic capacity in transgenic tobacco with reduced amounts of Rubisco

被引:130
作者
von Caemmerer, S [1 ]
Lawson, T
Oxborough, K
Baker, NR
Andrews, TJ
Raines, CA
机构
[1] Australian Natl Univ, Res Sch Biol Sci, Mol Plant Physiol Grp, Canberra, ACT 2601, Australia
[2] Univ Essex, Dept Biol Sci, Colchester CO4 3SQ, Essex, England
关键词
chlorophyll fluorescence; guard cell; Rubisco; stomatal conductance; tobacco;
D O I
10.1093/jxb/erh128
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
High-resolution imaging of chlorophyll a fluorescence from intact tobacco leaves was used to compare the quantum yield of PSII electron transport in the chloroplasts of guard cells with that in the underlying mesophyll cells. Transgenic tobacco plants with reduced amounts of Rubisco (anti-Rubisco plants) were compared with wild-type tobacco plants. The quantum yield of PSII in both guard cells and underlying mesophyll cells was less in anti-Rubisco plants than in wild-type plants, but closely matched between the two cell types regardless of genotype. CO2 assimilation rates of anti-Rubisco plants were 4.4 mumol m(-2) s(-1) compared with 17.3 mumol m(-2) s(-1) for the wild type, when measured at a photon irradiance of 1000 mumol m(-2) s(-1) and ambient CO2 of 380 mumol mol(-1). Despite the large difference in photosynthetic capacity between the anti-Rubisco and wild-type plants, there was no discernible difference in the rate of stomatal opening, steady-state stomatal conductance or response of stomatal conductance to ambient CO2 concentration. These data demonstrate clearly that the commonly observed correlation between photosynthetic capacity and stomatal conductance can be disrupted in the long term by manipulation of photosynthetic capacity via antisense RNA technology. It was concluded that stomatal conductance is not directly determined by the photosynthetic capacity of guard cells or the leaf mesophyll.
引用
收藏
页码:1157 / 1166
页数:10
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