Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae -: art. no. R72

被引：32

作者：

Boyer, J

Badis, G

Fairhead, C

Talla, E

Hantraye, F

Fabre, E

Fischer, G

Hennequin, C

Koszul, R

Lafontaine, I

Ozier-Kalogeropoulos, O

Ricchetti, M

Richard, GF

Thierry, A

Dujon, B

机构：

[1] Univ Paris 06, CNRS, URA 2171, Unite Genet Mol Levures, F-75724 Paris 15, France

[2] Univ Paris 06, UFR 927, F-75724 Paris 15, France

[3] Inst Pasteur, Dept Struct & Dynam Genomes, F-75724 Paris 15, France

[4] CNRS, Chim Bacterienne Lab, F-13402 Marseille 20, France

[5] Univ Paris 06, Parasitol Lab, F-75012 Paris, France

[6] Inst Pasteur, Unite Genet & Biochim Dev, F-75724 Paris 15, France

来源：

GENOME BIOLOGY | 2004年 / 5卷 / 09期

关键词：

D O I：

10.1186/gb-2004-5-9-r72

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes ( of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

引用

页数：19