Nanopore membrane-based electrochemical immunoassay

被引：9

作者：

Kim, Jinseong ^{[1
]}

Gonzalez-Martin, Anuncia ^{[1
]}

机构：

[1] Lynntech Inc, College Stn, TX 77840 USA

来源：

JOURNAL OF SOLID STATE ELECTROCHEMISTRY | 2009年 / 13卷 / 07期

基金：

美国国家卫生研究院;

关键词：

Electrochemical Immunoassay; Nanopore; Direct immunoassay; TRANSPORT-PROPERTIES;

D O I：

10.1007/s10008-009-0786-5

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Using nanotechnology in the immunoassay field, Lynntech has developed a nanopore-based sensor with electrochemical signal transduction for the detection of biologically relevant molecular targets. Antibodies of interest were immobilized on the inside wall of nanopores and the antibody-antigen interaction was monitored by measuring the ionic conductance through the nanopores. We have used electrochemical impedance spectroscopy to monitor the changes in the ionic conductance due to the antibody-antigen interaction. To aid the development of a portable, fast immunoassay instrument, we have selected specific impedance frequency values that are very sensitive to the ionic conductance changes. Biomarkers of prostate cancer, prostate-specific antigen and hepsin, were successfully assayed by the nanopore membrane-based electrochemical immunoassay in both phosphate-buffered saline and plasma medium.

引用

页码：1037 / 1042

页数：6

共 20 条

[1] A colony lift immunoassay for the specific identification and quantification of Listeria monocytogenes [J].