Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection

被引:30
作者
Lu, HT
Jiang, Y [1 ]
Li, HB
Chen, F
Wong, MH
机构
[1] Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China
[2] Univ Hong Kong, Dept Bot, Hong Kong, Peoples R China
[3] Hong Kong Baptist Univ, Croucher Inst Environm Sci, Kowloon, Hong Kong, Peoples R China
关键词
column liquid chromatography; fluorescence detection; tetracycline antibiotics; pharmaceuticals;
D O I
10.1365/s10337-004-0356-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C-18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 mug L-1, respectively. Data with respect to precision and accuracy were reported and discussed.
引用
收藏
页码:259 / 264
页数:6
相关论文
共 18 条
[1]   SERUM LEVELS OF TETRACYCLINE AFTER DIFFERENT ADMINISTRATION FORMS [J].
ALTMANN, AE ;
BEEUWKES, H ;
BROMBACHER, PJ ;
BUYTENDIJK, HJ ;
GIJZEN, AHJ ;
MAESEN, FPV .
CLINICA CHIMICA ACTA, 1968, 20 (02) :185-+
[2]  
Chen CL, 1995, J AOAC INT, V78, P1369
[3]   Validation of a high-performance liquid chromatography method for the determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline in bovine milk and muscle [J].
Cinquina, AL ;
Longo, F ;
Anastasi, G ;
Giannetti, L ;
Cozzani, R .
JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) :227-233
[4]   POSTCOLUMN ZIRCONIUM CHELATION AND FLUORESCENCE DETECTION FOR THE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TETRACYCLINES [J].
CROUBELS, S ;
BAEYENS, W ;
VANPETEGHEM, C .
ANALYTICA CHIMICA ACTA, 1995, 303 (01) :11-16
[5]   Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent [J].
Ding, XJ ;
Mou, SF .
JOURNAL OF CHROMATOGRAPHY A, 2000, 897 (1-2) :205-214
[6]   Quantitative analysis for oxytetracycline in medicated premixes and feeds by second-derivative synchronous spectrofluorimetry [J].
Fernandez-González, R ;
García-Falcón, MS ;
Simal-Gándara, J .
ANALYTICA CHIMICA ACTA, 2002, 455 (01) :143-148
[7]   Simplified liquid-chromatographic determination of residues of tetracycline antibiotics in eggs [J].
Furusawa, N .
CHROMATOGRAPHIA, 2001, 53 (1-2) :47-50
[8]   Determination of oxytetracycline in raw and cooked channel catfish by capillary electrophoresis [J].
Huang, TS ;
Du, WX ;
Marshall, MR ;
Wei, CI .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (07) :2602-2605
[9]   DETERMINATION OF TETRACYCLINE ANTIBIOTICS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].
IWAKI, K ;
OKUMURA, N ;
YAMAZAKI, M .
JOURNAL OF CHROMATOGRAPHY, 1992, 623 (01) :153-158
[10]   Evaluation of amperometric detection for the liquid-chromatographic determination of tetracycline antibiotics and their common contaminants in pharmaceutical formulations [J].
Kazemifard, AG ;
Moore, DE .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1997, 16 (04) :689-696