Directional neurite outgrowth and axonal differentiation of embryonic hippocampal neurons are promoted by a neurite outgrowth domain of the B2-chain of laminin

被引:41
作者
Matsuzawa, M
Weight, FF
Potember, RS
Liesi, P
机构
[1] NIAAA, MOL & CELLULAR NEUROBIOL LAB, ROCKVILLE, MD 20852 USA
[2] RIKEN, FRONTIER RES PROGRAM, WAKO, SAITAMA 35101, JAPAN
[3] JOHNS HOPKINS UNIV, APPL PHYS LAB, LAUREL, MD 21205 USA
关键词
neurite outgrowth; axonal differentiation; embryo; hippocampus; laminin;
D O I
10.1016/0736-5748(96)00014-7
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Molecular cues involved in directional neurite outgrowth and axonal differentiation of embryonic hippocampal neurons were studied on substrates coated in a striped 5 mu m pattern with synthetic peptides from a neurite outgrowth (RDIAEIIKDI, P1543) and cell attachment (CDPGYIGSR, P364) domain of the B2- and B1-chains of laminin, respectively. Both peptides supported neuronal attachment, but only the B2-chain-derived P1543 promoted expression of a mature neuronal phenotype. Directional neurite outgrowth and axonal differentiation of embryonic hippocampal neurons were selectively induced by striped substrates of the B2-chain-derived P1543. Axonal differentiation was determined by expression of a phosphorylated epitope of the 200 kDa neurofilament protein in the longer ''axonal'' neurite of the bipolar embryonic hippocampal neurons. Ethanol (100 mM), a neuroactive compound known to delay neuronal development, impaired both directional neurite outgrowth and expression of a phosphorylated epitope of the 200 kDa neurofilament protein on a patterned P1543 substratum. The present results provide direct evidence that a 10 amino acid peptide (P1543), derived from a neurite outgrowth domain of the B2-chain of laminin, may be an axonal guidance and differentiation factor for embryonic hippocampal neurons in vitro. Published by Elsevier Science Ltd.
引用
收藏
页码:283 / 295
页数:13
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