Determination of cocaine and its metabolites in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats

被引：19

作者：

Ma, F ^{[1
]}

Zhang, J ^{[1
]}

Lau, CE ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,DEPT PSYCHOL,NEW BRUNSWICK,NJ 08903

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 02期

关键词：

cocaine; norcocaine; benzoylecgonine; benzoylnorecgonine;

D O I：

10.1016/S0378-4347(97)00031-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating cocaine and its three metabolites in rat serum microsamples (50 mu l). The separation used a 2.1-mm I.D. reversed-phase Brownlee C-18 column with an isocratic mobile phase consisting of methanol-acetonitrile-25.8 mM sodium acetate buffer, pH 2.2, containing 1.29.10(-4) M tetrabutylammonium phosphate (12.5:10:77.5, v/v/v). The detection limit was 2.5 ng/ml for all the compounds using an ultraviolet detector operated at 235 nm. The method was used to study the pharmacokinetics of cocaine after an intravenous (i.v.) bolus dose (4 mg/kg).

引用

页码：307 / 312

页数：6

共 16 条

[1]

Barat SA, 1996, J APPL TOXICOL, V16, P215

[2] PROLONGED OCCURRENCE OF COCAINE IN HUMAN-SALIVA AND URINE AFTER CHRONIC USE [J].