Microwave fixation and localization of calcium in synaptic terminals using x-ray microanalysis and electron energy loss spectroscopy imaging

被引：13

作者：

Mizuhira, V

Hasegawa, H

机构：

[1] SHIONOGI & CO LTD, DEV RES LABS, TOYONAKA, OSAKA 561, JAPAN

[2] TOKYO MED & DENT UNIV, MED RES INST, BUNKYO KU, TOKYO 113, JAPAN

来源：

BRAIN RESEARCH BULLETIN | 1997年 / 43卷 / 01期

关键词：

microwave fixation; opening image; synaptic vesicles; calcium ion detection; computerized x-ray microanalysis; electron energy loss spectroscopy digital net Ca:L image;

D O I：

10.1016/S0361-9230(96)00428-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The distribution of calcium ions is demonstrated in synaptic terminals by means of a two-step chemical precipitation of calcium ions in the rat brain. K-oxalate/K-antimonate chemical replacement with simultaneous computerized microwave irradiation was used. This precipitate in nerve cell structures was investigated by computerized electron probe x-ray microanalysis (EDX) and electron energy loss spectroscopic (EELS) imaging. The values obtained by EDX agreed with those of the standard sample and theoretical values of Ca-antimonate. Typical EELS spectra of Ca:L, O:K, and Sb:M were obtained from nerve terminals in the same tissue block as that used for EDX analysis. Excellent net Ca:L and Sb:M EELS digital images were obtained after their background images were subtracted. Calcium ions were distributed in the nerve terminals, synaptic vesicles, mitochondria, and synaptic membranes. (C) 1997 Elsevier Science Inc.

引用

页码：53 / 58

页数：6

共 21 条

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