Arabidopsis DUO POLLEN3 Is a Key Regulator of Male Germline Development and Embryogenesis

被引：75

作者：

Brownfield, Lynette ^{[1
]}

Hafidh, Said ^{[1
]}

Durbarry, Anjusha ^{[1
]}

Khatab, Hoda ^{[1
]}

Sidorova, Anna ^{[1
]}

Doerner, Peter ^{[2
]}

Twell, David ^{[1
]}

机构：

[1] Univ Leicester, Dept Biol, Leicester LE1 7RH, Leics, England

[2] Univ Edinburgh, Sch Biol Sci, Inst Mol Plant Sci, Edinburgh EH9 3JH, Midlothian, Scotland

来源：

PLANT CELL | 2009年 / 21卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

MALE GAMETOPHYTE DEVELOPMENT; CELL-CYCLE; GENE-EXPRESSION; TUBE GUIDANCE; EGG CELL; SPERM; IDENTIFICATION; TRANSCRIPTOME; PROLIFERATION; FERTILIZATION;

D O I：

10.1105/tpc.109.066373

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Male germline development in angiosperms produces the pair of sperm cells required for double fertilization. A key regulator of this process in Arabidopsis thaliana is the male germline-specific transcription factor DUO POLLEN1 (DUO1) that coordinates germ cell division and gamete specification. Here, we uncover the role of DUO3, a nuclear protein that has a distinct, but overlapping role with DUO1 in male germline development. DUO3 is a conserved protein in land plants and is related to GON-4, a cell lineage regulator of gonadogenesis in Caenorhabditis elegans. Mutant duo3-1 germ cells either fail to divide or show a delay in division, and we show that, unlike DUO1, DUO3 promotes entry into mitosis independent of the G2/M regulator CYCB1;1. We also show that DUO3 is required for the expression of a subset of germline genes under DUO1 control and that like DUO1, DUO3 is essential for sperm cell specification and fertilization. Furthermore, we demonstrate an essential sporophytic role for DUO3 in cell division and embryo patterning. Our findings demonstrate essential developmental roles for DUO3 in cell cycle progression and cell specification in both gametophytic and sporophytic tissues.

引用

页码：1940 / 1956

页数：17

共 51 条

[1] GEX3, expressed in the male gametophyte and in the egg cell of Arabidopsis thaliana, is essential for micropylar pollen tube guidance and plays a role during early embryogenesis [J].