Early events during quiescent infection development by Colletotrichum gloeosporioides in unripe avocado fruits

被引:46
作者
Beno-Moualem, D [1 ]
Prusky, D [1 ]
机构
[1] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Prod, IL-50250 Bet Dagan, Israel
关键词
fruit resistance; preformed resistance;
D O I
10.1094/PHYTO.2000.90.5.553
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Inoculation of avocado pericarp tissue with Colletotrichum gloeosporioides and treatment of avocado cell cultures with the cell wall elicitor of C. gloeosporioides both increased the production of reactive oxygen species (ROS). However, whereas the production of ROS could be detected within minutes in avocado cell suspensions, it was detected only after 2 h following inoculation of pericarp tissue. Protein kinase inhibitors such as K-252a and staurosporine and the phosphatase inhibitor microcystin-LR inhibited the release of H(2)O(2) from avocado cell suspensions. When 1 mM H(2)O(2) was exogenously applied to pericarp tissue, it enhanced ROS, phenyl-alanine ammonia lyase (PAL) activity, and epicatechin levels. But, when H(2)O(2) treatment was applied following staurosporine treatment, PAL activity was no longer induced. The uninduced ROS production in pericarp tissue of freshly harvested, unripe, resistant fruit was twice as high as in ripe, susceptible fruit. Challenge inoculation of resistant fruit further increased the ROS level; however, this increase did not occur in susceptible fruits. The current findings are consistent with the hypothesis that production of ROS is induced by fungal infection of unripe fruits and, consequently, may modulate resistance, resulting in the inhibition of fungal development and quiescence.
引用
收藏
页码:553 / 559
页数:7
相关论文
共 53 条
[21]  
HE SY, 1993, CELL, V73, P1
[22]   INVOLVEMENT OF EPICATECHIN IN THE REGULATION OF LIPOXYGENASE ACTIVITY DURING ACTIVATION OF QUIESCENT COLLETOTRICHUM-GLOEOSPORIOIDES INFECTIONS OF RIPENING AVOCADO FRUITS [J].
KARNI, L ;
PRUSKY, D ;
KOBILER, I ;
BARSHIRA, E ;
KOBILER, D .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1989, 35 (04) :367-374
[23]   THE INITIATION OF MEMBRANE LIPID-PEROXIDATION DURING BACTERIA-INDUCED HYPERSENSITIVE REACTION [J].
KEPPLER, LD ;
NOVACKY, A .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1987, 30 (02) :233-245
[24]   PHYSIOLOGICAL-CHANGES IN SUSPENSION-CULTURED SOYBEAN CELLS ELICITED BY TREATMENT WITH CHITOSAN [J].
KOHLE, H ;
YOUNG, DH ;
KAUSS, H .
PLANT SCIENCE LETTERS, 1984, 33 (02) :221-230
[25]  
KONDO Y, 1993, DEV PLANT PATHOL, V2, P148
[26]  
LARSSON C, 1987, METHOD ENZYMOL, V148, P558
[27]   HYDROGEN-PEROXIDE STIMULATES SALICYLIC-ACID BIOSYNTHESIS IN TOBACCO [J].
LEON, J ;
LAWTON, MA ;
RASKIN, I .
PLANT PHYSIOLOGY, 1995, 108 (04) :1673-1678
[28]   H2O2 FROM THE OXIDATIVE BURST ORCHESTRATES THE PLANT HYPERSENSITIVE DISEASE RESISTANCE RESPONSE [J].
LEVINE, A ;
TENHAKEN, R ;
DIXON, R ;
LAMB, C .
CELL, 1994, 79 (04) :583-593
[29]   RAPID ELICITOR-INDUCED CHEMI-LUMINESCENCE IN SOYBEAN CELL-SUSPENSION CULTURES [J].
LINDNER, WA ;
HOFFMANN, C ;
GRISEBACH, H .
PHYTOCHEMISTRY, 1988, 27 (08) :2501-2503
[30]  
LISKER N, 1983, PHYSIOL PLANT PATHOL, V22, P331