DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin

被引：133

作者：

Huang, Haiping ^{[1
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci MOE, Nanjing 210093, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 25卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Aptamer; QDs; Electrochemiluminescence; Thrombin; NANOCRYSTAL QUANTUM DOTS; ELECTROGENERATED CHEMILUMINESCENCE; PROTEIN-DETECTION; BIND; MOLECULES; AMPLIFICATION; NANOPARTICLES; SELECTION; SEQUENCE; PROBE;

D O I：

10.1016/j.bios.2009.08.008

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel biosensor for the detection of thrombin was developed by using QDs electrochemiluminescence (ECL) technique. The thiol-terminated aptamer with 15 nucleotides (probe 1) was first immobilized on Au electrode, and then thrombin was imported to form the aptamer-thrombin bioaffinity complexes. Another 5'-biotin modified aptamer (29 nucleotides, probe 11) was next hybridized with the combined thrombin to form a sandwich type structure. Streptavidin modified QDs (avidin-QDs) were bound to probe 11 via the biotin-avidin-system. The QDs ECL signal was responsive to the amount of probe 11, which was indirect proportional to the combined thrombin. The ECL intensity of the biosensor increased with the increase of thrombin concentration in the range of 0-20 mu g mL(-1). In addition, the biosensor exhibited the excellent selectivity responses and good stability toward the target analyte. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：927 / 930

页数：4