Synthetic diamond films as a platform material for label-free protein sensors

被引：19

作者：

Bijnens, Nathalie ^{[1
,2
]}

Vermeeren, Veronique ^{[2
,3
]}

Daenen, Michael ^{[1
,2
]}

Grieten, Lars ^{[1
,2
]}

Haenen, Ken ^{[1
,2
,4
]}

Wenmackers, Sylvia ^{[1
,2
]}

Williams, Oliver A. ^{[1
,2
,4
]}

Ameloot, Marcel ^{[2
,3
]}

vandeVen, Martin ^{[2
,3
]}

Michiels, Luc ^{[2
,3
]}

Wagner, Patrick ^{[1
,2
]}

机构：

[1] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium

[2] Transnatl Univ Limburg, Sch Life Sci, B-3590 Diepenbeek, Belgium

[3] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium

[4] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium

来源：

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE | 2009年 / 206卷 / 03期

关键词：

C-REACTIVE PROTEIN; NANOCRYSTALLINE DIAMOND; IMPEDIMETRIC IMMUNOSENSORS; IMPEDANCE SPECTROSCOPY; DNA HYBRIDIZATION; THIN-FILMS; SURFACES; ELECTRODES; BIOSENSORS; SPR;

D O I：

10.1002/pssa.200880485

中图分类号：

T [工业技术];

学科分类号：

120111 [工业工程];

摘要：

In the framework of developing a fast and label-free immunosensor for C-reactive protein (CRP) detection, H-terminated nanocrystalline diamond (NCD) was functionalised with anti-CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real-time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC-labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 mu M CRP were recognised with a reaction time of 30 minutes. (C) 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：520 / 526

页数：7

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