Mass spectral analysis in proteomics

被引：210

作者：

Yates, JR ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2004年 / 33卷

关键词：

MALDI; ESI; tandem mass spectrometry; multidimensional separations;

D O I：

10.1146/annurev.biophys.33.111502.082538

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometry provides key tools for the analysis of proteins. New types of mass spectrometers that provide enhanced capability to discover protein identities and perform improved proteomic experiments are discussed. Handling the complex mixtures of peptides and proteins generated from protein complexes and whole cells requires multidimensional separations; several forms of separation are discussed. Applications of mass spectrometry-based approaches for contemporary proteomic analyses are described.

引用

页码：297 / 316

页数：20

共 92 条

[1] A new algorithm for the evaluation of shotgun peptide sequencing in proteomics: Support vector machine classification of peptide MS/MS spectra and SEQUEST scores [J].

Anderson, DC ;

Li, WQ ;

Payan, DG ;

Noble, WS .

JOURNAL OF PROTEOME RESEARCH, 2003, 2 (02) :137-146

[2]

Bienvenut WV, 2002, PROTEOMICS, V2, P868, DOI 10.1002/1615-9861(200207)2:7<868::AID-PROT868>3.0.CO

[3]

2-D

[4] Enrichment of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry [J].

Blonder, J ;

Goshe, MB ;

Moore, RJ ;

Pasa-Tolic, L ;

Masselon, CD ;

Lipton, MS ;

Smith, RD .

JOURNAL OF PROTEOME RESEARCH, 2002, 1 (04) :351-360

[5] SEQUENCE-SPECIFIC FRAGMENTATION OF MATRIX-ASSISTED LASER-DESORBED PROTEIN PEPTIDE IONS [J].

BROWN, RS ;

LENNON, JJ .

ANALYTICAL CHEMISTRY, 1995, 67 (21) :3990-3999

[6] Selective detection and sequencing of phosphopeptides at the femtomole level by mass spectrometry [J].

Carr, SA ;

Huddleston, MJ ;

Annan, RS .

ANALYTICAL BIOCHEMISTRY, 1996, 239 (02) :180-192

[7]

CARR SA, 1993, PROTEIN SCI, V2, P183

[8] An interface with a linear quadrupole ion guide for an electrospray-ion trap mass spectrometer system [J].

Cha, BC ;

Blades, M ;

Douglas, DJ .

ANALYTICAL CHEMISTRY, 2000, 72 (22) :5647-5654

[9]

Chaurand P, 2002, ELECTROPHORESIS, V23, P3125, DOI 10.1002/1522-2683(200209)23:18<3125::AID-ELPS3125>3.0.CO

[10]

2-#

← 1 2 3 4 5 6 7 8 9 10 →