Sequential kinetic resolution catalyzed by halohydrin dehalogenase

被引：37

作者：

Elenkov, Maja Majeric

Tang, Lixia

Hauer, Bernhard

Janssen, Dick B.

机构：

[1] Univ Groningen, Biochem Lab, Groningen Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands

[2] BASF AG, Fine Chem & Biocatalysis Res, D-6700 Ludwigshafen, Germany

来源：

ORGANIC LETTERS | 2006年 / 8卷 / 19期

关键词：

D O I：

10.1021/ol061369h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A sequential kinetic resolution catalyzed by halohydrin dehalogenase was employed for the synthesis of two valuable enantiopure building blocks. Resolution of methyl 4-chloro-3-hydroxybutanoate methylester ((R,S)-2) with use of a Trp249Phe mutant of halohydrin dehalogenase yielded methyl 4-cyano-3-hydroxybutanoate methylester ((S)-4) with 96.8% ee (40% yield) and (S)-2 with 95.2% ee (41% yield). This reaction is carried out in aqueous solution under mild conditions and provides access to a useful statin side-chain building block.

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收藏

页码：4227 / 4229

页数：3

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