Dominant T cells in idiopathic nephrotic syndrome of childhood

被引:39
作者
Frank, C
Herrmann, M
Fernandez, S
Dirnecker, D
Böswald, M
Kolowos, W
Ruder, H
Haas, JP
机构
[1] Univ Erlangen Nurnberg, Lab Pediat Immunol & Rheumatol, Childrens Hosp, D-91054 Erlangen, Germany
[2] Univ Erlangen Nurnberg, Inst Clin Immunol, D-91054 Erlangen, Germany
[3] Childrens Rheumatol Hosp, Garmisch Partenkirchen, Germany
关键词
childhood nephrotic syndrome; CDR3 length polymorphism; cell receptor; immune system; focal global sclerosis;
D O I
10.1046/j.1523-1755.2000.00870.x
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Background. Because of several studies, idiopathic nephrotic syndrome (INS) of childhood is suspected to have an immunologic pathogenesis with T cells playing a major role. To investigate this hypothesis further, we studied the diversity of the CDR3 region of the T-cell receptor (TCR) beta-chain from peripheral T cells isolated from patients with INS. Methods. The study was performed over a three-year period to obtain longitudinal data on the repertoire of peripheral T cells, mRNA from peripheral mononuclear cells (PBMCs) of seven INS patients and two healthy controls (NHD) was prepared and analyzed for CDR3 length polymorphism of TCR beta-chain by spectratyping. Results. All INS patients presented individually skewed spectratype histograms in at least one V beta-family. Patients suffering from a frequent relapsing course of INS or a focal global sclerosis showed some alterations to persist in all samples isolated in the observation period (up to 3 years). In addition, sequence analyses of the beta-chain of the TCR CDR3 region confirmed clonal expansion of peripheral T cells in those patients who had displayed spectratype alterations. Conclusions. The data give strong evidence for an direct involvement of CD8+ T cells in the complicated course of INS.
引用
收藏
页码:510 / 517
页数:8
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