A universal procedure for primer labelling of amplicons

被引：46

作者：

Neilan, BA

Wilton, AN

Jacobs, D

机构：

[1] Sch. of Microbiology and Immunology, University of New South Wales, Sydney

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 14期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1093/nar/25.14.2938

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection and visualisation of nucleic acids is integral to genome analyses. Exponential amplification procedures have provided the means for the manipulation of nucleic acid sequences, which were otherwise inaccessible. We describe the development and application of a universal method for the labelling of any PCR product using a single end-labelled primer. Amplification was performed in a single reaction with the resulting amplicon labelled to a high specific activity. The method was adapted to a wide range of PCRs and significantly reduced the expense of such analyses.

引用

页码：2938 / 2939

页数：2

共 5 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

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