Proteome analysis of human follicular fluid

被引:118
作者
Angelucci, Stefania
Ciavardelli, Domenico
Di Giuseppe, Fabrizio
Eleuterio, Enrica
Sulpizio, Marilisa
Tiboni, Gian Mario
Giampietro, Franca
Palumbo, Paola
Di Ilio, Carmine
机构
[1] Univ G dAnnunzio, Fac Med, Dept Biomed Sci G Annunzio, I-66013 Chieti, Italy
[2] G Annunzio Univ Fdn, Ctr Excellence Aging, Chieti, Italy
[3] Univ G dAnnunzio, Dept Med & Aging, Chieti, Italy
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2006年 / 1764卷 / 11期
关键词
Proteome analysis; human follicular fluid; folliculogenesis; acute-phase response protein;
D O I
10.1016/j.bbapap.2006.09.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We used proteomic approach to analyze the protein profile of human follicular fluid (HFF) obtained from 25 non-no-ovulatory women undergoing assisted reproduction techniques due to a male infertility factor. In all HFF samples analyzed we found 695 common spots distributed in the 3 to 10 pH range and in the 10-200 kDa range. Only 625 of these spots were also present in the plasma. We used MALDI-TOF-MS analysis to unequivocally assign 183 HFF/plasma matched spots and 27 HFF/plasma unmatched spots. A large number of acute-phase proteins, including transferrin, ceruloplasmin, afamin, hemopexin, haptoglobin and plasma amyloid protein, were identified in HFF in relatively high concentration supporting the hypothesis that mammalian ovulation can be compared to an inflammatory event. We also identified several important antioxidant enzymes; i.e., catalase, superoxide dismutase, glutathione transferase, paraoxonase, heat shock protein 27 and protein disulfide isomerase. This indicates that during maturation the human follicle is well protected against toxic injury due to oxidative stress. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1775 / 1785
页数:11
相关论文
共 62 条
[1]  
Adiga SK, 2002, EUR J OBSTET GYN R B, V105, P150
[2]   Purification of apolipoprotein H(beta2-glycoprotein I)-like protein from human follicular fluid [J].
Aleporou-Marinou, V ;
Pappa, H ;
Yalouris, P ;
Patargias, T .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2001, 128 (03) :537-542
[3]  
Anahory T, 2002, ELECTROPHORESIS, V23, P1197, DOI 10.1002/1522-2683(200204)23:7/8<1197::AID-ELPS1197>3.0.CO
[4]  
2-2
[5]   OVARIAN VITAMIN-E ACCUMULATION - EVIDENCE FOR A ROLE OF LIPOPROTEINS [J].
ATEN, RF ;
KOLODECIK, TR ;
BEHRMAN, HR .
ENDOCRINOLOGY, 1994, 135 (02) :533-539
[6]   Glutathione S-transferases in human ovarian follicular fluid [J].
Bisseling, JGA ;
Knapen, MFCM ;
Goverde, HJM ;
Mulder, TPJ ;
Peters, WHM ;
Willemsen, WNP ;
Thomas, CMG ;
Steegers, EAP .
FERTILITY AND STERILITY, 1997, 68 (05) :907-911
[7]   ISOLATION AND CHARACTERIZATION OF A NEW PLACENTA SPECIFIC PROTEIN (PP12) [J].
BOHN, H ;
KRAUS, W .
ARCHIVES OF GYNECOLOGY, 1980, 229 (04) :279-291
[8]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9]   Transferrin in the developing ovarian follicle: evidence for de-novo expression by granulosa cells [J].
Briggs, DA ;
Sharp, DJ ;
Miller, D ;
Gosden, RG .
MOLECULAR HUMAN REPRODUCTION, 1999, 5 (12) :1107-1114
[10]   EVIDENCE FOR THE BINDING OF HUMAN-SERUM AMYLOID P COMPONENT TO CLQ AND FAB-GAMMA [J].
BRISTOW, CL ;
BOACKLE, RJ .
MOLECULAR IMMUNOLOGY, 1986, 23 (10) :1045-1052