Evanescent wave-initiated photopolymerisation as a new way to create monolithic open-tubular capillary columns: use as enzymatic microreactor for on-line protein digestion

被引：32

作者：

Abele, Silvija ^{[2
,3
]}

Smejkal, Petr ^{[1
,2
]}

Yavorska, Oksana ^{[2
]}

Foret, Frantisek ^{[1
]}

Macka, Mirek ^{[2
]}

机构：

[1] Inst Analyt Chem ASCR, Vvi, Brno 60200, Czech Republic

[2] Dublin City Univ, Sch Chem Sci, Dublin 9, Ireland

[3] Univ Latvia, Fac Chem, LV-1586 Riga, Latvia

来源：

ANALYST | 2010年 / 135卷 / 03期

关键词：

POROUS POLYMER MONOLITHS; STATIONARY PHASES; SEPARATION MEDIA; PHOTOINITIATED POLYMERIZATION; LIQUID-CHROMATOGRAPHY; ZONE-ELECTROPHORESIS; FUSED-SILICA; ELECTROCHROMATOGRAPHY; LAYER; TRYPSIN;

D O I：

10.1039/b920789a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Evanescent wave-initiated photopolymerisation in an optically wave guiding PTFE-coated fused silica capillary using light-emitting diode as a light source, is established here as a way to fabricate monolithic porous layer open-tubular capillary columns with a potential in capillary separation methods; application of the obtained open-tubular columns as enzymatic microreactors for on-line protein digestion is demonstrated.

引用

页码：477 / 481

页数：5

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