Study on the production of a xylanolytic complex from Penicillium canescens 10-10c

被引:24
作者
Gaspar, A [1 ]
Cosson, T [1 ]
Roques, C [1 ]
Thonart, P [1 ]
机构
[1] LESAFFRE DEV,F-59706 MARCQ EN BAROEUL,FRANCE
关键词
Penicillium canescens 10-10c; xylanases; fed-batch culture; hydrodynamic stress;
D O I
10.1007/BF02787840
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We screened about a hundred microorganisms (including unidentified yeasts, fungi, and bacteria) for their ability to produce xylanolytic enzymes. About 40 of them were hemicellulolytic; among these, we selected Penicillium canescens 10-10c for detailed study because of its ability to produce an interesting enzymatic complex in quantity. The xylanase complex was cellulase-free, and had an optimal activity at pH 4.6-5.0 and 55-60 degrees C on birchwood xylan. The best production was on soya meal and wheat straw; expression of the xylanase was repressed by glucose, xylose, and lactose. The optimization of culture medium and mode (fed-batch) enabled us to improve the production three to four times. The importance of the mixing conditions on the biomass and xylanase production is also reported.
引用
收藏
页码:45 / 58
页数:14
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