Gas chromatographic separation and mass spectrometric identification of mixtures of oxyphytosterol and oxycholesterol derivatives - Application to a phytosterol-enriched food

被引：71

作者：

Grandgirard, A ^{[1
]}

Martine, L ^{[1
]}

Joffre, C ^{[1
]}

Juaneda, P ^{[1
]}

Berdeaux, O ^{[1
]}

机构：

[1] INRA, Unite Nutr Lipid, F-21065 Dijon, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1040卷 / 02期

关键词：

food analysis; oxyphytosterols; oxycholesterols; oxysterols; sterols;

D O I：

10.1016/j.chroma.2004.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pure individual phytosterols were prepared using reversed-phase HPLC in order to obtain the oxidized compounds of sitosterol, campesterol, stigmasterol and brassicasterol. 7-Hydroxy-, 7-keto-, 5,6-epoxy-, 40-hydroxy-, 4-ene-6-hydroxy-, 6-keto- and 5alpha,6beta-dihydroxyphytosterols were obtained as well as analogous compounds of cholesterol. The gas chromatographic properties as well as the electronic impact mass spectra of these compounds (as trimethylsilyl ether derivatives) were studied. These data were used to identify oxyphytosterols in a spread enriched in phytosterols: the oxyphytosterols represented no more than 68 mug/g of spread (about 0.08% of phytosterols were oxidised). (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：239 / 250

页数：12

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[43] GC-MS CHARACTERIZATION AND QUANTIFICATION OF STEROLS AND CHOLESTEROL OXIDATION-PRODUCTS [J].