Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization

被引：261

作者：

Concepcion, Joy ^{[1
]}

Witte, Krista ^{[1
]}

Wartchow, Charles ^{[1
]}

Choo, Sae ^{[1
]}

Yao, Danfeng ^{[1
]}

Persson, Henrik ^{[1
]}

Wei, Jing ^{[1
]}

Li, Pu ^{[1
]}

Heidecker, Bettina ^{[1
]}

Ma, Weilei ^{[1
]}

Varma, Ram ^{[1
]}

Zhao, Lian-She ^{[1
]}

Perillat, Donald ^{[1
]}

Carricato, Greg ^{[1
]}

Recknor, Michael ^{[1
]}

Du, Kevin ^{[1
]}

Ho, Huddee ^{[1
]}

Ellis, Tim ^{[1
]}

Gamez, Juan ^{[1
]}

Howes, Michael ^{[1
]}

Phi-Wilson, Janette ^{[1
]}

Lockard, Scott ^{[1
]}

Zuk, Robert ^{[1
]}

Tan, Hong ^{[1
]}

机构：

[1] ForteBio Inc, Menlo Pk, CA 94025 USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2009年 / 12卷 / 08期

关键词：

SURFACE-PLASMON RESONANCE; BIACORE TECHNOLOGY; SENSORS; BINDING; LIGANDS; DNA;

D O I：

10.2174/138620709789104915

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.

引用

页码：791 / 800

页数：10

共 23 条

[1] Comparative analyses of a small molecule/enzyme interaction by multiple users of Biacore technology [J].

Cannon, MJ ;

Papalia, GA ;

Navratilova, I ;

Fisher, RJ ;

Roberts, LR ;

Worthy, KM ;

Stephen, AG ;

Marchesini, GR ;

Collins, EJ ;

Casper, D ;

Qiu, HW ;

Satpaev, D ;

Liparoto, SF ;

Rice, DA ;

Gorshkova, II ;

Darling, RJ ;

Bennett, DB ;

Sekar, M ;

Hommema, E ;

Liang, AM ;

Day, ES ;

Inman, J ;

Karlicek, SM ;

Ullrich, SJ ;

Hodges, D ;

Chu, T ;

Sullivan, E ;

Simpson, J ;

Rafique, A ;

Luginbühl, B ;

Westin, SN ;

Bynum, M ;

Cachia, P ;

Li, YJ ;

Kao, D ;

Neurauter, A ;

Wong, M ;

Swanson, M ;

Myszka, DG .

ANALYTICAL BIOCHEMISTRY, 2004, 330 (01) :98-113

[2]

Cooper Matthew A, 2005, Drug Discov Today Technol, V2, P241, DOI 10.1016/j.ddtec.2005.08.014

[3]

CUATRECASEAS P, 1972, BIOCHEMISTRY-US, V11, P291

[4]

FEIGLIN M, 1999, J BIOMOLECULAR SCEEN, V4, P169

[5] MONITORING OF PROTEINS IN HYBRIDOMA CULTURES BY HPLC [J].

GAILLARD, I ;

MARTIAL, A ;

PINTON, H ;

MARC, A ;

ENGASSER, JM ;

FICK, M .

BIOTECHNOLOGY TECHNIQUES, 1994, 8 (04) :215-220

[6]

GARDNER A R, 1989, Biotechnology Techniques, V3, P401, DOI 10.1007/BF01875008

[7] CHEMICAL AND BIOCHEMICAL SENSORS BASED ON INTERFEROMETRY AT THIN (MULTI-)LAYERS [J].

GAUGLITZ, G ;

BRECHT, A ;

KRAUS, G ;

NAHM, W .

SENSORS AND ACTUATORS B-CHEMICAL, 1993, 11 (1-3) :21-27

[8] ZERO-LENGTH CROSSLINKING PROCEDURE WITH THE USE OF ACTIVE ESTERS [J].

GRABAREK, Z ;

GERGELY, J .

ANALYTICAL BIOCHEMISTRY, 1990, 185 (01) :131-135

[9]

Harlow E., 1988, Antibodies: A Laboratory Manual

[10]

HERMANSON GT, 1996, BIOCONJUGATE TECHNIQ

← 1 2 3 →