MONITORING OF PROTEINS IN HYBRIDOMA CULTURES BY HPLC

被引：1

作者：

GAILLARD, I

MARTIAL, A

PINTON, H

MARC, A

ENGASSER, JM

FICK, M

机构：

[1] Laboratoire des Sciences du Génie Chimique, Institut National Polytechnique de Lorraine, CNRS UP 6811, Vandoeuvre Cedex, 54505, 2, avenue de la Forêt de Haye

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 04期

关键词：

D O I：

10.1007/BF00155410

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid, accurate and flexible analysis of protein concentrations, and notably IgG concentrations, in crude hybridoma culture media is essential to provide production optimization. An analytical method using two chromatographic columns has been optimized in order to monitor proteins in the course of several cultures and, consequently, to establish the evolution of their concentrations with rapidity and reliability.

引用

页码：215 / 220

页数：6

共 6 条

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