Watching DNA Condensation Induced by Poly(amido amine) Dendrimers with Time-Resolved Cryo-TEM

被引：36

作者：

Carnerup, Anna M. ^{[1
]}

Ainalem, Marie-Louise ^{[1
]}

Alfredsson, Viveka ^{[1
]}

Nylander, Tommy ^{[1
]}

机构：

[1] Lund Univ, Div Phys Chem, Ctr Chem & Chem Engn, S-22100 Lund, Sweden

来源：

LANGMUIR | 2009年 / 25卷 / 21期

基金：

芬兰科学院;

关键词：

POLYAMIDOAMINE DENDRIMERS; CRYOELECTRON MICROSCOPY; MULTIVALENT CATIONS; MOLECULAR-DYNAMICS; LIGHT-SCATTERING; FINE-STRUCTURE; NUCLEATION; PACKING; SURFACE; GROWTH;

D O I：

10.1021/la903068v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The condensation of DNA by poly(amido amine) dendrimers of generation 1, 2, and 4 has been followed by time-resolved cryogenic transmission electron microscopy (cryo-TEM). The recorded images show that significant morphological rearrangement occurs for DNA condensed with the lower generation dendrimers leading to the formation of toroidal aggregates. Higher charge density dendrimers, oil the other hand, give rise to globular aggregates, where no transient morphologies are observed. We suggest that the dendrimers in this case are kinetically trapped as soon as they bind to the DNA strand.

引用

页码：12466 / 12470

页数：5

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