Imaging of small molecules in tissue sections with a new intermediate-pressure MALDI linear ion trap mass spectrometer

被引：117

作者：

Garrett, Timothy J.

Prieto-Conaway, Maria C.

Kovtoun, Viatcheslav

Bui, Huy

Izgarian, Nick

Stafford, George

Yost, Richard A.

机构：

[1] Univ Florida, Dept Chem, Gainesville, FL 32611 USA

[2] Thermo Electron Corp, San Jose, CA USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2007年 / 260卷 / 2-3期

基金：

美国国家卫生研究院;

关键词：

imaging MS; MALDL intermediate pressure; linear ion trap; phospholipids;

D O I：

10.1016/j.ijms.2006.09.019

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

We describe a new intermediate-pressure MALDI linear ion trap mass spectrometer and its capabilities for imaging mass spectrometry. The instrument design is described and is characterized in terms of four performance issues (1) MALDI performance at intermediate pressure; (2) analysis of samples on non-conductive and conductive glass slides; (3) critical importance of tandem mass spectrometry (both MS2 and MS3) for identification of analyte species and imaging of isobaric species; (4) capability for repeated analysis of the same tissue section. Application of the new instrument to imaging phospholipids in rat brain sections is described in detail. (C) 2006 Elsevier B.V. All rights reserved.

引用

页码：166 / 176

页数：11

共 36 条

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