The influence of nicotinic receptor subunit composition upon agonist, α-bungarotoxin and insecticide (imidacloprid) binding affinity

被引:109
作者
Lansdell, SJ [1 ]
Millar, NS [1 ]
机构
[1] UCL, Wellcome Lab Mol Pharmacol, Dept Pharmacol, London WC1E 6BT, England
基金
英国惠康基金;
关键词
nicotinic receptor; insecticide binding; imidacloprid; epibatidine; alpha-bungarotoxin;
D O I
10.1016/S0028-3908(99)00170-7
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A series of cell lines stably expressing recombinant nicotinic acetylcholine receptors (nAChRs) has been established by transfection of mammalian (rat) and insect (Drosophila) nicotinic subunit cDNAs. By equilibrium radioligand binding, we have examined the influence of individual subunits upon the affinity of two nicotinic agonists (epibatidine and methylcarbamylcholine), an antagonist (the snake neurotoxin, alpha-bungarotoxin) and a recently developed chloronicotinyl insecticide (imidacloprid). Imidacloprid bound with very low affinity to the rat alpha 4/beta 2 nAChR but did so with high affinity to hybrid nACRs containing Drosophila a subunits co-assembled with rat beta 2. Of the subunit combinations examined, imidacloprid showed highest affinity binding to nAChRs containing the recently identified Drosophila a subunit, D alpha 3, co-assembled with beta 2. In contrast, no specific binding of imidacloprid was detected when Da3 was co-expressed with the mammalian neuronal beta 4 subunit, or with the muscle-type (gamma or delta) subunits. However, despite the absence of imidacloprid binding to D alpha 3/beta 4, D alpha 3/gamma or D alpha 3/delta, these subunit combinations all exhibited high affinity binding of other nicotinic radioligands. Epibatidine showed substantially higher affinity binding to subunit combinations containing neuronal (beta 2 or beta 4) subunits than it did to combinations containing muscle-type (gamma or delta) subunits. In contrast, alpha-bungarotoxin bound with higher affinity to combinations containing muscle-type subunits. Our results demonstrate that both alpha and non-alpha subunits exert a profound influence upon the affinity of nicotinic ligands for recombinant nAChRs. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:671 / 679
页数:9
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