Solvent partitioning and whole-cell sitosterol bioconversion activity in aqueous-organic two-phase systems

被引：28

作者：

Cruz, A

Fernandes, P

Cabral, JMS

Pinheiro, HM

机构：

[1] Inst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal

[2] Univ Lusofona Humanidades & Tecnol, P-1749024 Lisbon, Portugal

[3] Biotrend, P-2675807 Ramada, Portugal

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2004年 / 34卷 / 3-4期

关键词：

sterol side-chain cleavage; biphasic systems; Mycobacterium sp; whole-cell biocatalysis; phthalate solvents;

D O I：

10.1016/j.enzmictec.2003.11.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The combined effect of solvent nature and aqueous medium composition on the efficiency of a whole-cell biocatalyst in organic solvent-containing systems was evaluated, using the side-chain cleavage of beta-sitosterol by free mycobacterial cells as model system. Trials were performed in two-phase liquid systems and in solvent-saturated aqueous systems. Aqueous phase composition influenced solvent distribution between the cells and the medium and, hence, catalytic activity. The resulting partition coefficient was different for active and autoclaved cells, suggesting active solvent incorporation. This coefficient was used to estimate the solvent concentration in the cells at system saturation and led to the identification of systems for which high solvent concentration in the cells apparently favoured catalytic activity. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：342 / 353

页数：12

共 39 条

[1] Lipophilic compounds in biotechnology - interactions with cells and technological problems [J].