Aligned carbon nanotube thin films for DNA electrochemical sensing

被引:36
作者
Berti, F. [1 ]
Lozzi, L. [2 ]
Palchetti, I. [1 ]
Santucci, S. [2 ]
Marrazza, G. [1 ]
机构
[1] Univ Florence, Dept Chem, I-50019 Florence, Italy
[2] Univ Aquila, Dept Phys, I-67100 Laquila, Italy
关键词
CNT; CVD; DNA; Genosensor; DPV; HYBRIDIZATION; ELECTRODES; BIOSENSORS; SENSORS;
D O I
10.1016/j.electacta.2009.01.038
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Carbon nanotubes are interesting materials for DNA electrochemical sensing due to their unique electric properties: high surface area, fast heterogeneous electron transfer, and electrochemical stability. In this work aligned Carbon NanoTube (CNT) thin films were designed and tested as candidate platforms for DNA immobilization and for the development of an electrochemical genosensor. The films were prepared by Chemical Vapor Deposition (CVD) using acetylene and ammonia as precursor gases and nickel particles as catalyst. A preliminary electrochemical characterization was performed using cyclic voltammetry since, so far, these films have been used only for gas sensing. The surfaces were then covalently functionalized with a DNA oligonucleotide probe, complementary to the sequence of the most common inserts in the GMOs: the Promoter 35S. The genosensor format involved the immobilization of the probe onto the sensor surface, the hybridization with the target-sequence and the electrochemical detection of the duplex formation. Careful attention was paid to the probe immobilization conditions in order to minimize the signal due to non-specifically adsorbed sequences. For the detection of the hybridization event both label-free and enzyme-labelled methods were investigated. In case of the enzyme-labelled method a target concentration at nanomolar level can be easily detected, with a linear response from 50 nM to 200 nM, whereas the label-free method showed a linear response between 0.5 mu M and 10 mu M. The reproducibility was 11% and 20% with the enzyme-labelled method and the label-free method, respectively. The batch-to-batch reproducibility of the different sensors was also evaluated. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5035 / 5041
页数:7
相关论文
共 34 条
[1]  
Bard A. J., 2001, ELECTROCHEMICAL METH
[2]   Carbon nanotubes - the route toward applications [J].
Baughman, RH ;
Zakhidov, AA ;
de Heer, WA .
SCIENCE, 2002, 297 (5582) :787-792
[3]   Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection [J].
Cai, H ;
Cao, XN ;
Jiang, Y ;
He, PG ;
Fang, YZ .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 375 (02) :287-293
[4]   A review of DNA functionalized/grafted carbon nanotubes and their characterization [J].
Daniel, Sobhi ;
Rao, Talasila Prasada ;
Rao, Kota Sreenivasa ;
Rani, Sikhakolli Usha ;
Naidu, G. R. K. ;
Lee, Hea-Yeon ;
Kawai, Tomoji .
SENSORS AND ACTUATORS B-CHEMICAL, 2007, 122 (02) :672-682
[5]   Carbon nanotube synthesized on metallic substrates [J].
Emmenegger, C ;
Mauron, P ;
Züttel, A ;
Nützenadel, C ;
Schneuwly, A ;
Gallay, R ;
Schlapbach, L .
APPLIED SURFACE SCIENCE, 2000, 162 :452-456
[6]   Direct DNA hybridization at disposable graphite electrodes modified with carbon nanotubes [J].
Erdem, Arzum ;
Papakonstantinou, Pagona ;
Murphy, Hayley .
ANALYTICAL CHEMISTRY, 2006, 78 (18) :6656-6659
[7]   Disposable electrochemical genosensor for the simultaneous analysis of different bacterial food contaminants [J].
Farabullini, F. ;
Lucarelli, F. ;
Palchetti, I. ;
Marrazza, G. ;
Mascini, M. .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) :1544-1549
[8]   Aligned carbon nanotube-DNA electrochemical sensors [J].
He, PG ;
Dai, LM .
CHEMICAL COMMUNICATIONS, 2004, (03) :348-349
[9]   HELICAL MICROTUBULES OF GRAPHITIC CARBON [J].
IIJIMA, S .
NATURE, 1991, 354 (6348) :56-58
[10]   Detection of achondroplasia G380R mutation from PCR amplicons by using inosine modified carbon electrodes based on electrochemical DNA chip technology [J].
Kara, P ;
Ozkan, D ;
Erdem, A ;
Kerman, K ;
Pehlivan, S ;
Ozkinay, F ;
Unuvar, D ;
Itirli, G ;
Ozsoz, M .
CLINICA CHIMICA ACTA, 2003, 336 (1-2) :57-64