Electron movement along DNA strands: Use of intercalators and electron paramagnetic resonance spectroscopy

被引:30
作者
Pezeshk, A
Symons, MCR
McClymont, JD
机构
[1] DE MONTFORT UNIV,DEPT APPL SCI,LEICESTER LE1 9BH,LEICS,ENGLAND
[2] UNIV LEICESTER,DEPT CHEM,LEICESTER LE1 7RH,LEICS,ENGLAND
关键词
D O I
10.1021/jp960821j
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Exposure of aqueous solutions of the sodium salt of DNA to ionizing radiation at 77 K gives trapped electron-loss and electron-gain centers in the stacked bases that were well characterized by EPR spectroscopy. We find that when mitoxanthrone and AQ(4) are intercalated, the yields of the signals from the electron-gain centers are greatly reduced, being replaced by the EPR spectra of the intercalator radical anions. Fifty percent capture was observed for average intercalator separation of ca. 110 base pairs. At this concentration, the mean distance traveled by the ejected electrons prior to any type of capture is ca. 31 base pairs. These results are discussed in terms of their possible relevance to cellular DNA, with respect to both radiation damage and drug action. They are also compared with extensive work of others in this general area.
引用
收藏
页码:18562 / 18566
页数:5
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