Down-regulated miR-9 and miR-433 in human gastric carcinoma

被引：161

作者：

Luo, Hongchun ^{[1
]}

Zhang, Hongbin ^{[2
]}

Zhang, Zhenzhen ^{[3
]}

Zhang, Xia ^{[1
]}

Ning, Bo ^{[1
]}

Guo, Jinjun ^{[1
]}

Nie, Na ^{[4
]}

Liu, Bo ^{[4
]}

Wu, Xiaoling ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, Chongqing 400010, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Dept Hematol, Chongqing 400016, Peoples R China

[3] Chongqing Med Univ, Viral Hepatitis Res Inst, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China

[4] Chongqing Med Univ, Dept Lab Med, Chongqing 400016, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH | 2009年 / 28卷

关键词：

TUMOR-SUPPRESSOR; MICRORNAS; PROSTATE; GENES; RNAS;

D O I：

10.1186/1756-9966-28-82

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors. Methods: To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot. Results: We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively. Conclusion: Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.

引用

页数：9

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