Biodistribution of TNF-α-coated gold nanoparticles in an in vivo model system

被引：101

作者：

Goel, Raghav ^{[1
]}

Shah, Neha ^{[1
]}

Visaria, Rachana ^{[2
]}

Paciotti, Giulio F. ^{[3
]}

Bischof, John C. ^{[1
,2
,4
]}

机构：

[1] Univ Minnesota, Dept Biomed Engn, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Mech Engn, Minneapolis, MN 55455 USA

[3] CytImmune Sci Inc, Rockville, MD USA

[4] Univ Minnesota, Sch Med, Dept Urol Surg, Minneapolis, MN 55455 USA

来源：

NANOMEDICINE | 2009年 / 4卷 / 04期

关键词：

biodistribution; cryosurgery; CYT-6091; gold nanoparticle; TNF; TUMOR-NECROSIS-FACTOR; COLLOIDAL GOLD; THERMAL THERAPY; PARTICLE-SIZE; CANCER; DELIVERY; NANOTECHNOLOGY; RAT;

D O I：

10.2217/NNM.09.21

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: In this study, we describe the biodistribution of CYT-6091, a colloidal gold (Au)-based nanomedicine that targets the delivery of TNF-alpha to solid tumors. Materials & methods: A single intravenous injection of CYT-6091 coated with 5 mu g TNF-alpha was given to human prostate tumor-bearing or naive (without tumor) nude mice. Tissues were harvested and analyzed at specific time points for Au nanoparticles by atomic emission spectroscopy and TNF-alpha by ELISA. Results: The two constituents of CYT-6091, TNF-alpha and Au, exhibited different behavior in blood, with TNF-alpha showing a faster decay than the Au nanoparticles. Between 0 and 4 h after injection, TNF-alpha showed a preferential accumulation in the tumor. Au was observed to accumulate preferentially in the liver between 4 and 12 h, and showed some clearance over time (4 months). Conclusion: These data suggest that CYT-6091 delivers TNF-alpha preferentially to the tumor and that upon TNF-alpha degradation, the liver takes up Au, which is cleared slowly over time.

引用

页码：401 / 410

页数：10

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