Protein microarray detection strategies: focus on direct detection technologies

被引：129

作者：

Espina, V

Woodhouse, EC

Wulfkuhle, J

Asmussen, HD

Petricoin, EF

Liotta, LA

机构：

[1] US FDA, NCI, Clin Proteom Program, Pathol Lab,Ctr Canc Res, Bethesda, MD 20892 USA

[2] Univ Colorado, Boulder, CO 80309 USA

[3] US FDA, Ctr Biol Evaluat & Res, NCI, Clin Proteom Program, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2004年 / 290卷 / 1-2期

关键词：

protein microarray; catalyzed reporter deposition; molecular profiling; fluorescence; chromogens;

D O I：

10.1016/j.jim.2004.04.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein microarrays are being utilized for functional proteomic analysis, providing information not obtainable by gene arrays. Microarray technology is applicable for studying protein-protein, protein-ligand, kinase activity and posuranslational modifications of proteins. A precise and sensitive protein microarray, the direct detection or reverse-phase microarray, has been applied to ongoing clinical trials at the National Cancer Institute for studying phosphorylation events in EGF-receptor-mediated cell signaling pathways. The variety of microarray applications allows for multiple, creative microarray designs and detection strategies. Herein, we discuss detection strategies and challenges for protein microarray technology, focusing on direct detection of protein microarrays. Published by Elsevier B.V.

引用

页码：121 / 133

页数：13

共 90 条

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