Deoxyribonucleic acid (DNA) biosensors for environmental risk assessment and drug studies

被引:118
作者
Bagni, Graziana [1 ]
Osella, Domenico
Sturchio, Elena
Mascini, Marco
机构
[1] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Firenza, Italy
[2] Univ Piemonte Orientale, Dept Environm & Life Sci, I-15100 Alessandria, Italy
[3] Natl Author Hlth & Safety Work ISPESL, Dept Ind Installat & Interact Environm, DIPIA, I-00184 Rome, Italy
关键词
DNA-based biosensors; environmental risk assessment; DNA damage; anti-proliferative drugs;
D O I
10.1016/j.aca.2006.03.085
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In the present work, electrochemical DNA biosensors are proposed as a screening device for the rapid bio-analysis of environmental pollution and DNA-drug interaction studies. The binding of small molecules to DNA immobilised on disposable screen-printed electrodes has been measured through the variation of the electrochemical signal of guanine by square wave voltammetric scans. These kinds of biosensors were used to evaluate the soil contamination level in an Italian polluted area and the results were compared with several methods for the DNA damage detection, as Comet genotoxicity effects, aberrant anatelophases and micronucleated cells frequency on plant roots, and with fixed wavelength fluorescence (FF) by using 2-aminoanthracene as standard compound. The results showed the ability of the biosensors to distinguish in 11 min low, medium and high contaminated soils with good correlation with well established techniques as well as FF, Comet and genotoxicity tests. The same kind of biosensors was also used to evaluate the interaction of DNA with some anti-proliferative metallo drugs, and the electrochemical responses reflected the kind of interaction. The reproducibility of the electrochemical measurements of DNA guanine peak was estimated as, less than 10% of relative standard deviation (R.S.D.%). (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:81 / 89
页数:9
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