Identification of variant translocations in chronic myeloid leukemia by fluorescence in situ hybridization

被引：8

作者：

Acar, H

Stewart, J

Boyd, E

Conner, MJ

机构：

[1] UNIV SELCUK,DEPT MED GENET,KONYA,TURKEY

[2] ROYAL HOSP SICK CHILDREN,DEPT MED GENET,GLASGOW G21 8SJ,LANARK,SCOTLAND

来源：

CANCER GENETICS AND CYTOGENETICS | 1997年 / 93卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(96)00168-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We studied two cases of chronic myeloid leukemia (CML) having variant complex translocations detected by trypsin G-banding and fluorescence in situ hybridization (FISH). Application of dual color- (DC-) FISH using abl and bcr cosmid probes permitted us to detect the bcr-abl fusion event on both interphase nuclei and metaphase spread. Furthermore, FISH using combinatorial hybridization (centromeric-library and library-library probes) demonstrated the content and the position of the translocations in CML patients with variant (complex type) Ph-positive rearrangements. FISH analysis appears to be superior than conventional cytogenetic analysis. (C) Elsevier Science Inc., 1997.

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收藏

页码：115 / 118

页数：4

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