Esr proteins are secreted by the cells of the embryo surrounding region

被引:47
作者
Bonello, JF
Sevilla-Lecoq, S
Berne, A
Risueño, MC
Dumas, C
Rogowsky, PM
机构
[1] Ecole Normale Super Lyon, UCBL, CNRS, UMR 5667,INRA, F-69364 Lyon 07, France
[2] CSIC, Ctr Invest Biol, Org Nucl Desarrollo Plantas, E-28006 Madrid, Spain
关键词
endosperm; immunohistochemisty; ligand; protein-protein interaction; Zea mays;
D O I
10.1093/jxb/erf010
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Three highly homologous Esr genes are expressed specifically in the embryo surrounding legion at the micropylar end of the maize endosperm. The proteins belong to a family of small hydrophilic proteins that share a conserved motive with CIv3, the ligand of the receptor-like kinase CIv1. In this study, co-localization of Esr proteins with their mRNAs in the embryo surrounding region was shown with polyclonal antibodies recognizing all three Esr proteins. On a subcellular level the secretion of Esr proteins and their association with the cell wall was shown independently by cell fractionation, immunohistochemistry and transient expression of Gfp fusion proteins. Furthermore, a possible interaction of Esr proteins with a 35 kDa protein present in the lower half of maize kernels was suggested by in vitro affinity chromatography. Therefore Esr proteins share two characteristics with ligands of receptor-like kinases: they are released in the extracellular space and they have the capacity to form protein-protein interactions.
引用
收藏
页码:1559 / 1568
页数:10
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