The effect of nitrogen application on the grain yield and grain protein concentration of barley was studied in 13 field trials covering a wide range of soil N conditions over 4 years at locations in south-eastern Queensland. The main objectives of the study were to quantify the response of barley to N application rate over a range of environmental conditions, and to explain the response in terms of soil mineral N, total N uptake, and N distribution in the plants. Barley made efficient use of N (60 kg grain/kg N) until grain yield reached 90% of maximum yield. Grain protein concentration did not increase to levels unacceptable for malting purposes until grain yield exceeded 85-90% of maximum yield. Nitrogen harvest index was generally high (above 0.75), and did not decrease until the total N supply exceeded that necessary for maximum grain yield. Rates of application of N for malting barley should be determined on the basis of soil analysis (nitrate-N) to 1 m depth and 90% of expected maximum grain yield, assuming that 17 kg N is taken up per tonne of grain produced. It can further be assumed thar, the crop makes full use of the nitrate N to Im present at planting, provided the soil is moist to 1 m. A framework relating grain yield to total N uptake, N harvest index, and grain N concentration is presented. Further, total N uptake of fertilised barley is related to N uptake without fertiliser, fertiliser application rate, and apparent N recovery. The findings reported here will be useful in the development of barley simulation models and decision support packages that can be used to aid N management.