Statistical accuracy in fluorescence fluctuation experiments

被引：80

作者：

Kask, P ^{[1
]}

Gunther, R ^{[1
]}

Axhausen, P ^{[1
]}

机构：

[1] EVOTEC BIOSYST GMBH,D-22529 HAMBURG,GERMANY

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 1997年 / 25卷 / 03期

关键词：

fluorescence correlation spectroscopy; fluorescence intensity fluctuations; fluorescence intensity distribution analysis;

D O I：

10.1007/s002490050028

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The mathematical expression of the signal to noise ratio in fluorescence fluctuation experiments is derived for arbitrary sample profiles and for any mechanism of translational motion, and experimentally verified. The signal to noise ratio depends on the mean count rate per particle per dwell time, the mean number of particles per sample volume, time characteristics of the correlation function, sample profile characteristics, and the data collection time. Statistical accuracy of the third order moment of fluorescence intensity fluctuations is also studied. The optimum concentration for the third order moment analysis is about one particle per sample volume.

引用

页码：163 / 169

页数：7

共 8 条

[1] FLUORESCENCE CORRELATION SPECTROSCOPY .1. CONCEPTUAL BASIS AND THEORY [J].